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M94A2706.TXT
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1994-10-25
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Document 2706
DOCN M94A2706
TI Field comparison of line-immunoassays with western blot.
DT 9412
AU Maskill W; Tamashiro H; Belsey E; M'Boup S; Wasi C; Liomba G;
Gershy-Damet G; Fairfield University Melbourne.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):243 (abstract no. PB0401). Unique
Identifier : AIDSLINE ICA10/94369869
AB OBJECTIVE: To determine under field conditions the accuracy of
line-immunoassays (LIA's) for confirming seropositivity for HIV-1 and
HIV-2 as compared to the Western Blot (WB). METHODS: Sera from four
countries which were according to WHO WB interpretation criteria
positive for HIV-1 (n = 650), HIV-2 (n = 35), both HIV-1 and HIV-2 (n =
63), negative (n = 571) or indeterminate (n = 47) were tested by two
LIA's (Inno-Lia and Pepti-Lav). Results of the LIA's were interpreted
according to the criteria provided by the test manufacturers. RESULTS:
The Inno-Lia and Pepti-Lav tests showed a 98.3% and 98.2% agreement
respectively with the WB for HIV-1 positive sera. Both LIA's showed 100%
agreement with the WB for HIV-2 positive sera. Both assays showed
approximately only a 60% agreement with the WB for sera positive for
both HIV-1 and HIV-2 with the remaining sera being positive only for
HIV-1. The LIA's found the majority of WB indeterminate sera to be
negative, however both tests showed poor specificity due to WB negative
sera showing indeterminate reactions. DISCUSSION AND CONCLUSION:
Although the Inno-Lia and Pepti-Lav tests show a high level of agreement
of WB positive sera, find the majority of WB indeterminate samples
non-reactive, and produce a lower number of dually reactive sera, both
assays produce a significant number of indeterminate reactions with WB
negative sera. The occurrence of these indeterminate reactions may limit
the ability of these tests to completely replace the WB.
DE AIDS Serodiagnosis/*METHODS *Blotting, Western Comparative Study
Human HIV Antibodies/BLOOD HIV Seropositivity/*DIAGNOSIS/IMMUNOLOGY
HIV-1/*IMMUNOLOGY HIV-2/*IMMUNOLOGY *Immunoassay Predictive Value of
Tests MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).